Aspartate Amino transferase (AST/GOT) Activity Assay Kit (Reitman-Frankel Method)

Detection Principle:
AST enables alpha-ketoglutaric acid and aspartic acid to displace amino to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
Instrument: Spectrophotometer
Assay Time: 100 min

Tested Sample Types: Serum, plasma, animal tissue

Research Area's: Enzymes,Liver Biomarkers

Shelf Life: 6 Months

Storage:
2-8℃