Asymmetric Dimethylarginine (ADMA) Detection Kit

Use: Used to detect the concentration of asymmetric dimethylarginine (ADMA) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled asymmetric dimethylarginine (ADMA) is used, and the purified anti-asymmetric dimethylarginine (ADMA) antibody is coated on the microplate. In the competitive inhibition reaction, a certain amount of solid-phase antibody and biotin-labeled asymmetric dimethylarginine (ADMA) and non-labeled antigen (calibrator or specimen) inhibit the competitive reaction. The binding amount of antibody and biotin-labeled asymmetric dimethylarginine (ADMA) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of antibody and biotin-labeled asymmetric dimethylarginine (ADMA), and vice versa. After the reaction is balanced, solid-phase antibody-biotinylated asymmetric dimethylarginine (ADMA) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated asymmetric dimethylarginine (ADMA)-enzyme-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of asymmetric dimethylarginine (ADMA) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation, and rapidity, and has reliable detection performance for the reduction or increase of asymmetric dimethylarginine (ADMA) in the sample.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.