ATF1 polyclonal, anti-human

Background:
Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1. ATF-1 binds the cAMP response element (CRE) and mediates PKA-induced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro.

Alternative Name:
cAMP-dependent transcription factor ATF-1, ATF1, Activating transcription factor 1, ATF 1, atf1, ATF1 EWS fusion gene, ATF1 FUS fusion gene, ATF1, cAMP dependent transcription factor 1, cAMP-dependent transcription factor ATF-1, Cyclic AMP dependent transcription factor ATF 1, Cyclic AMP dependent transcription factor ATF1, Cyclic AMP-dependent transcription factor ATF-1, EWS AFT1, FUS ATF 1, FUS/ATF 1, Protein TREB36, RNA binding protein activating transcription factor 1 fusion protein, TREB 36, TREB36, TREB36 protein,

Application Dilution:
WB:1,500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: ATF1 polyclonal antibody detects endogenous levels of ATF1 protein.

Immunogen:
recombinant protein

MW: 29 kDa

Swiss-Prot: P18846(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.