ATF4 Polyclonal Antibody

Background:
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
ATF4, CREB-2, CREB2, TAXREB67, TXREB

Immunogen: Recombinant fusion protein of human ATF4 (NP_001666.2).

Swissprot: P18848
Gene ID: 468

Calculated MW: 38 kDa
Observed MW: 50 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000 IF 1:50-1:200"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Citation(s):
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0194310