Atg12 polyclonal, anti-human

Background:
Atg12 (autophagy-related protein 12), also known as APG12, APG12L, FBR93 or HAPG12, is a 140 amino acid protein that is ubiquitously expressed and belongs to the Atg12 family of proteins. Atg12 is a homolog of the yeast protein Apg12 that participates in autophagy. Autophagy is a membrane trafficking mechanism that delivers cytoplasmic cargo to the vacuole/lysosome for degradation and recycling. In yeast, autophagy requires a protein conjugation system consisting of Apg12 covalently bound at the carboxy terminal glycine to lysine 149 of Apg5. Similarly in humans, Atg12 is essential for autophagy and localizes to the cytoplasm where it is covalently bound to APG5, a conjugation reaction that requires APG7, Atg10 and ATP. The Atg12-APG5 conjugate functions as an important regulator of the autophagic process and is required for the change in membrane morphology and development of autophagosomes. Due to alternative splicing events, two Atg12 isoforms exist.

Alternative Name:
APG12-like, ATG12, APG12, APG12L, APG12-like, APG12L, ATG12, ATG12 autophagy related 12 homolog (S. cerevisiae), ATG12 autophagy related 12 homolog, ATG12, Autophagy 12, Autophagy-related protein 12, FBR93, HAPG12, Ubiquitin-like protein ATG12,

Application Dilution:
WB:1:500-1:2,000
IHC:1:50-1:200

Specificity: Atg12 polyclonal antibody detects endogenous levels of Atg12 protein.

Immunogen:
Recombinant protein within human Atg12 aa 1-100.

MW: 8/15 kDa

Swiss-Prot: O94817(Human)

Purification & Purity:
Protein affinity purified.

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.