Atg4B monoclonal, anti-human, mouse, rat

Description: Anti-Atg4B Rabbit Monoclonal Antibody . Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Protein Function:
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C- terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms..

Other Names: Cysteine protease ATG4B, 3.4.22.-, AUT-like 1 cysteine endopeptidase, Autophagy-related cysteine endopeptidase 1, Autophagin-1, Autophagy-related protein 4 homolog B, HsAPG4B, hAPG4B, ATG4B {ECO:0000303|PubMed:15187094, ECO:0000312|HGNC:HGNC:20790}

Clone Name(s):
Clone: ABIA-1

Subcellular Localization: Cytoplasm.

Tissue Specificity: Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas..

Gene ID: 23192

Immunogen: A synthesized peptide derived from human Atg4B

Calculated MW: 44294

Purification: Affinity-chromatography

Format: Liquid

Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Concentration (mg/ml): Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.