Aurora B polyclonal, anti-human

Background:
Aurora related kinase-1 (ARK-1, STK15, Aurora2, Aik1) and -2 (ARK-2, STK12, Aurora1) are centrosome-associated serine/ threonine kinases that regulate centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis. ARK-1 and -2 are expressed in the nucleus and localize to distinct portions of mitotic machinery such as the centrosome, spindle poles (ARK-1), and midbody (ARK-2) during mitosis. ARK-1 and -2 transcripts are present at high levels in human thymus and fetal liver. ARK-1 protein has elevated expression in colon carcinoma lines (HT-29, SNU-C2B, COLO 205, SW480, 837 and 948) and accumulates during metaphase in HeLa cells. ARK-2 protein levels are maximal during both S and G2/M phases, whereas ARK-1 protein is degraded after G2/M via the ubiquitin-proteasome pathway. ARK-2 has a unique genetic loci relative to ARK-1, suggesting that these two kinases, with oncogenic potential, have different roles in cell cycle progression.

Alternative Name:
AIM-1, ARK-2, Aurora-related kinase 2, AURKB, AIK2, AIM-1, AIM1, ARK-2, ARK2, AurB, AURKB, AURKB, Aurora 1, Aurora and Ipl1 like midbody associated protein 1, Aurora kinase B, Aurora related kinase 2, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora-related kinase 2, Aurora/IPL1 related kinase 2, Aurora/IPL1-related kinase 2, IPL1, PPP1R48, Protein phosphatase 1 regulatory subunit 48, Serine/theronine kinase 12, Serine/threonine protein kinase 12, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase aurora-B, STK-1, STK1, STK12, STK5,

Application Dilution:
WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: Aurora B polyclonal antibody detects endogenous levels of Aurora B protein.

Immunogen:
recombinant protein

MW: 39 kDa

Swiss-Prot: Q96GD4(Human) O70126(Mouse) O55099(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.