Aurora B polyclonal, anti-rat, mouse

Quantity: 100 µg

Background:
AURKB(aurora kinase B) also called Aik2, AIM-1, ARK2, AurB, STK12, IPL1, PPP1R48 or STK5, localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. Cell cycle and Northern blot analyses showed that STK12 is expressed in the S phase and persistently thereafter. Western blot analysis indicated that STK12 is localized in the midbodies during anaphase. Northern blot analysis detected strong expression of a 1.5-kb STK12 transcript in thymus, with weaker expression in small intestine, testis, colon, spleen, and brain. The AURKB gene is mapped on 17p13.1. Examination of the role of both kinases in the phosphorylation of CENPA revealed that the reaction is mediated sequentially by AURKA and AURKB in early mitosis. EB1 overexpression enhanced AURKB kinase activity, and knockdown of EB1 with small interfering RNA impaired AURKB activity. EB1 protected AURKB from dephosphorylation/inactivation by protein phosphatase-2A by blocking binding of PP2A to AURKB. 

Description:
Rabbit IgG polyclonal antibody for Aurora kinase B (AURKB) detection. Tested with WB in Mouse, Rat.

Synonyms:
AIK2 antibody, AIM-1 antibody, AIM1 antibody, ARK-2 antibody, ARK2 antibody, AurB antibody, AURKB antibody, AURKB_HUMAN antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody, Aurora kinase B antibody, Aurora related kinase 2 antibody, Aurora- and Ipl1-like midbody-associated protein 1 antibody, Aurora-B antibody, Aurora-related kinase 2 antibody, Aurora/IPL1 related kinase 2 antibody, Aurora/IPL1-related kinase 2 antibody, IPL1 antibody, Serine/theronine kinase 12 antibody, Serine/threonine protein kinase 12 antibody, Serine/threonine-protein kinase 12 antibody, Serine/threonine-protein kinase aurora-B antibody, STK-1 antibody, STK1 antibody, STK12 antibody, STK5 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Aurora B(72-87aa QNKQPFTIDNFEIGRP), different from the related rat sequence by two anino acids.A synthetic peptide corresponding to a sequence at the N-terminus of mouse Aurora B(72-87aa QNKQPFTIDNFEIGRP), different from the related rat sequence by two anino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: rat.
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.