β-actin Mouse mAb

Background:
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.

Cellular localization: Cytoplasm,Cytoskeleton

Specificity: Human

Alternative Names:
actin antibody, b-actin antibody, beta-actin antibody

Immunogen: Synthetic Peptide

Isotype: Mouse Monoclonal IgG1

Concentration: 1mg/ml

Recommended dilutions: WB 1:5,000-10,000, IHC 1:200, IF 1:200

Observed band: 43kDa

GeneID: 60

Swiss-Prot No.: P60709

Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Store at -20°C. Avoid repeated freeze-thaw cycles.

NOTE: For Research Use Only