B Blood Group (clone BGRL 1) Affinity Purified, anti-human

Decription:
Mouse monoclonal antibody to human Blood Group B Affinity purified. The histo-blood group B antigen is defined by the carbohydrate structure at the non-reducing termini of oligosaccharide chains of glycoproteins and glycolipids. Carbohydrate chains are synthesized by the action of alpha -D-galactosyltransferase, which catalyzes the transfer of D-galactose monosaccharide to an acceptor substrate, the H antigen. The structure of the B antigen is : Gal (alpha 1-3)Gal(beta 1-3)GlcNAc-R-Fuc (alpha 1-2).

Synonyms: Blood Group B antigen

Clone: BGRL 1

Isotype: Mouse IgG1 kappa light chain

Immunogen: Blood group B erythrocytes

Reactivity: human

Form: Affinity purified, in 0.1 M glycine buffer, 1 M Tris buffer, pH 7.4, sterile filtered

Applications: Flow Cytometry, Indirect Immunofluorescence, Immunoblotting, Immunocytochemical techniques.

Working Dilution: Optimal dilution should be performed by user

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
1. Anstee, DJ & Cartron, J-P (1997) In: Applications of molecular biology to transfusion medicine, 17-49 (Garratty, G editor).
American Association of Blood Banks, Bethesda, MD.
2. Daniels, G. (1995) Human Blood Groups. Blackwell Science, Oxford
3. Issitt, PD & Anstee, DJ (1998) Applied Blood Group Serology. 4th edition. Montgomery Scientific Publications, Durhamm, NC
4. Mollison, PL. et al. (1997) Blood Transfusion in Clinical Medicine. 10th edition. Blackwell Science, Oxford
5. Reid, ME & Lomas-Francis, C. (1997) The Blood Group Antigen Facts Book. Academic Press, London
6. Chester, MA et al. (1990) Proceedings of the second international workshop and symposium on monoclonal antibodies against human red blood cells and related antigens, Lund
6. Curtis, BR et al. (2000) Blood group A and B antigens are strongly expressed on platelets of some individuals. Blood 96: 1574-1581