β-NGF monoclonal, anti-human
€0.00
In stock
SKU
AC-ABG10524
Catalog Number: AC-ABG10524
Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
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Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
Request Information
Source:
Produced in mice using highly pure recombinant human beta-NGF as the immunizing antigen. This IgG2b kappa antibody was purified from cell culture by Protein G affinity chromatography.
Western Blot:
To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 200 pg/ml of recombinant Human beta-NGF when used with BioGems biotinylated antigen affinity purified anti-Human beta-NGF (60-247BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
Immunohistochemistry:
This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 2.5 μg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. See photo(s). Optimal concentrations and conditions may vary.
Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
Information and photo are courtesy of Flagship Biosciences, LLC / Histology Services.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human beta-NGF (10.0 ng/ml), a concentration of 2.7 - 4.0 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced in mice using highly pure recombinant human beta-NGF as the immunizing antigen. This IgG2b kappa antibody was purified from cell culture by Protein G affinity chromatography.
Western Blot:
To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 200 pg/ml of recombinant Human beta-NGF when used with BioGems biotinylated antigen affinity purified anti-Human beta-NGF (60-247BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
Immunohistochemistry:
This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 2.5 μg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. See photo(s). Optimal concentrations and conditions may vary.
Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
Information and photo are courtesy of Flagship Biosciences, LLC / Histology Services.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human beta-NGF (10.0 ng/ml), a concentration of 2.7 - 4.0 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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