B Raf polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
BRAF(v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf, also known as ONCOGENE BRAF, BRAF1, RAFB1, Proto-oncogene B-Raf p94, v-Raf murine sarcoma viral oncogene homolog B1, proto-oncogene B-Raf, v-Raf murine sarcoma viral oncogene homolog B1, while the protein is more formally known as serine/threonine-protein kinase B-Raf. BRAF deduced 651-amino acid protein has a calculated molecular mass of 72.5 kD. Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, fetal brain, and placenta and transcripts of 2.6 and 4.5 kb in testis. Testis also showed lower expression of the 10- and 13-kb transcripts.B-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion.

Description:
Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase B-raf (BRAF) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
FLJ95109 antibody, 94 kDa B raf protein antibody, B raf 1 antibody, B Raf proto oncogene serine threonine protein kinase antibody, BRAF 1 antibody, BRAF antibody, BRAF_HUMAN antibody, BRAF1 antibody, cRmil antibody, MGC126806 antibody, MGC138284 antibody, Murine sarcoma viral(v-raf) oncogene homolog B1 antibody, Murine sarcoma viral v raf oncogene homolog B1 antibody, NS7 antibody, p94 antibody, Proto-oncogene B-Raf antibody, Proto-oncogene c-Rmil antibody, RAFB 1 antibody, RAFB1 antibody, Serine/threonine-protein kinase B-raf antibody, v raf murine sarcoma viral oncogene homolog B1 antibody, v-Raf murine sarcoma viral oncogene homolog B1 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human B Raf(344-362aa QPFRPADEDHRNQFGQRDR), identical to the related rat and mouse sequences.A synthetic peptide corresponding to a sequence in the middle region of human B Raf(344-362aa QPFRPADEDHRNQFGQRDR), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.