β-Tubulin Mouse mAb

Background:
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

Specificity: Human

Immunogen: Synthetic Peptide

Isotype: Mouse Monoclonal IgG1

Concentration: 1mg/ml

Recommended dilutions: WB 1:5,000-20,000, IHC 1:200, IF 1:200

Observed band: 55kDa

GeneID: 203068

Swiss-Prot No.: P07437

Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Store at -20°C. Avoid repeated freeze-thaw cycles.

NOTE: For Research Use Only