Basic Hair Keratin K81 (hHb1) polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB3 and KRTHB6, is found primarily in the hair cortex. Mutations in this gene and KRTHB6 have been observed in patients with a rare dominant hair disease, monilethrix.

Synonyms:
KRT81, HB1, Hb-1, KRTHB1, MLN137, ghHkb1, hHAKB2-1

Isotype: Guinea pig Polyclonal (GP-hHb1.2)

Immunogen:
Synthetic peptide of human hair (trichocytic) keratin K81 (formerly designated keratin hHb1; C- GISSLGVGSCGSS), conjugated to KLH

Form: Stabilized Serum

Specificity:
The antiserum stains specifically human hair keratin K81 present in the hair cortex and hair cuticle and tumors derived therefrom.

Reactivity: human, mouse

Applications: Immunohistochemistry on frozen and paraffin sections after trypsin and microwave treatment, Immunoblotting

Working DIlution: 1:200 for immunohistochemistry; 1:2,000 for immunoblotting (ECL method)

Storage: 2-8C

References:
- Langbein L. et al. (1999). J Biol Chem 274: 19874 – 19893
- Langbein L. (2004) Methods in Cell Biology 78: 413-456
- Langbein L, Schweizer J (2005) Intern Rev Cytology 243: 1-78