Basic Hair Keratin K82 (hHb2) polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB3 and KRTHB6, is found primarily in the hair cortex. Mutations in this gene and KRTHB6 have been observed in patients with a rare dominant hair disease, monilethrix.

Synonyms:
KRT82, HB2, Hb-2, KRTHB2, hHB2

Isotype: Guinea pig Polyclonal (GP-hHb2)

Immunogen:
Synthetic peptide of human hair (trichocytic) keratin K82 (formerly designated keratin hHb2, SIV GTG ELY VPC EPQ GLL SC), conjugated to KLH

Form: Stabilized Serum

Specificity:
The antiserum stains specifically human hair keratin K82 present starting from the mid bulb region in the hair cuticle (contrary to K32, expression starting from the lower part of the cuticle).

Reactivity: human, mouse

Applications: Immunohistochemistry on frozen sections, Immunoblotting

Working DIlution: 1:200 for immunohistochemistry; 1:2,000 for immunoblotting (ECL method)

Storage: 2-8C

References:
- Langbein, L. et al. (1999) J. Biol. Chem. 274: 19874-19893
- Langbein L et al. (2004) Methods in Cell V Biology 78: 413-456