Basic Hair Keratin K84 (hHb4) polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB3 and KRTHB6, is found primarily in the hair cortex. Mutations in this gene and KRTHB6 have been observed in patients with a rare dominant hair disease, monilethrix.

Synonyms:
KRT84, HB4, Hb-4, KRTHB4, hHb4

Isotype: Guinea pig Polyclonal (GP-hHb4)

Immunogen:
Synthetic peptide of human hair (trichocytic) keratin hHb4 (IAA VGS RPI HCG VRF), conjugated to KLH

Form: Stabilized Serum

Specificity:
The antiserum stains specifically human hair keratin K84 expressed starting from the upper layers of the filiform papillae of tongue epithelium and in mouse tail buds. No staining of anagen hair follicle.

Reactivity: human, mouse

Applications: Immunohistochemistry on frozen sections, Immunoblotting

Working DIlution: 1:200 for immunohistochemistry; 1:2,000 for immunoblotting (ECL method)

Storage: 2-8C

References:
- Langbein, L. et al. (1999) J. Biol. Chem. 274: 19874-19893
- Langbein L et al. (2004) Methods in Cell V Biology 78: 413-456