Basic Hair Keratin K86 (hHb6) polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB3 and KRTHB6, is found primarily in the hair cortex. Mutations in this gene and KRTHB6 have been observed in patients with a rare dominant hair disease, monilethrix.

Synonyms:
KRT86, HB6, Hb-6, KRTHB6, hHb6

Isotype: Guinea pig Polyclonal (GP-hHb6)

Immunogen:
Synthetic peptide of human basic hair (trichocytic) keratin K86 (formerly also designated hHb6; APV VST RVS SVP SNS NVV VGT TNA C), coupled to KLH

Form: Stabilized Serum

Specificity:
The antiserum stains specifically human hair keratin K86 expressed starting from the middle hair cortex. It can be defect in Monilethrix.

Reactivity: human, mouse

Applications: Immunohistochemistry on frozen sections, Immunoblotting

Working DIlution: 1:200 for immunohistochemistry, 1:2,000 for immunoblotting (ECL method)

Storage: 2-8C

References:
- Langbein, L. et al. (1999) J. Biol. Chem. 274: 19874-19893
- Langbein L et al. (2004) Methods in Cell V Biology 78: 413-456