Bence Jones kappa (clone NI 285, NI 250), anti-human

Background:
The reactivity of this preparation of two monoclonal antibodies is restricted to a surface determinant on the kappa light chain. It is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding enzyme immunoassay, immunoblotting,

immunoprecipitation and direct immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidase staining and immunoblotting. The optimum working dilution is an assayrelated characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Clone: NI 285, NI 250

Isotype: Mouse IgG1

Source: Highly purified Bence Jones kappa proteins isolated from pooled human urine.

Reactivity: human. The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.

Specificity: Monoclonal Mouse anti-serum to Human Ig kappa chain, free and bound

Form: Delipidated, heat inactivated, lyophilized, stable whole ascites. No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration 1.0 mg/ml. No foreign proteins added.

Applications: Indirect immunofluorescence, ELISA

Storage: Store at 4C, or in small aliquots at -20C.