Bence Jones lambda (clone NI 412, NI 268), anti-human

Background:
The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100; in ELISA from 1:100 upwards; in Western blotting from 1:200 upwards. These data should be interpreted as general recommendations only.

Clone: NI 412, NI 268

Isotype: Mouse IgG1

Source: Highly purified Bence Jones lambda proteins isolated from pooled human urine.

Reactivity: human. The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.

Specificity: Biotin-conjugated purified monoclonal Mouse antibody to Human Ig lambda chain, free and bound

Form: No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.