beta Actin polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
beta-Actin(ACTB) also known as BETA-ACTIN or ACTIN, CYTOPLASMIC, 1, is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al.(1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped 4 ACTB pseudogenes to other chromosomes. Karakozova et al.(2006) found that arginylation of beta-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al.(2006) concluded that arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.

Description:
Rabbit IgG polyclonal antibody for Actin, cytoplasmic 1 (ACTB) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
A26C1A antibody, A26C1B antibody, ACTB antibody, ACTB_HUMAN antibody, Actin cytoplasmic 1 antibody, Actx antibody, b-actin antibody, beta cytoskeletal actin antibody, Beta-actin antibody, E430023M04Rik antibody, MGC128179 antibody, PS1TP5 binding protein 1 antibody, PS1TP5BP1 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin(2-19aaDDDIAALVVDNGSGMCKA), identical to the related rat and mouse sequences.A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin(2-19aaDDDIAALVVDNGSGMCKA), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.