bFGF-147 human active recombinant protein
€295.00
In stock
SKU
600112
Protein Family: Cytokines, CAM ligands
Pathway and Disease: Signal Transduction, Cancers, Cell Motility, Cell Growth and Death
Alternate Names: Heparin-binding growth factor 2, HBGF-2, Basic fibroblast growth factor, BFGF, Prostatropin, FGF2, FGFBDescription:
Basic fibroblast growth factor (bFGF or Heparin-binding growth factor 2) is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor. Recombinant Human FGF-b (FGF-2) isoform 147 produced in E. coli is a single non-glycosylated polypeptide chain containing 147 amino acids with a MW of 16,539 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Recombinant Human Fibroblast Growth Factor-beta is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells is 0.12 ng/ml.
Accession No.: P09038
MW: 16539 Da
Sequence:
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Purity:
Purity > 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 50 ug protein lyophilized in 10 mM sodium phosphate pH8.0. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Signal Transduction, Cancers, Cell Motility, Cell Growth and Death
Alternate Names: Heparin-binding growth factor 2, HBGF-2, Basic fibroblast growth factor, BFGF, Prostatropin, FGF2, FGFBDescription:
Basic fibroblast growth factor (bFGF or Heparin-binding growth factor 2) is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor. Recombinant Human FGF-b (FGF-2) isoform 147 produced in E. coli is a single non-glycosylated polypeptide chain containing 147 amino acids with a MW of 16,539 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Recombinant Human Fibroblast Growth Factor-beta is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells is 0.12 ng/ml.
Accession No.: P09038
MW: 16539 Da
Sequence:
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Purity:
Purity > 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 50 ug protein lyophilized in 10 mM sodium phosphate pH8.0. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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