bFGF human active recombinant protein
€295.00
In stock
SKU
600113
Protein Family: Cytokines, CAM ligands
Pathway and Disease: Signal Transduction, Cancers, Cell Motility, Cell Growth and Death
Alternate Names: Heparin-binding growth factor 2, HBGF-2, Basic fibroblast growth factor, BFGF, Prostatropin, FGF2, FGFB, FGF-2, FGF-b 154Description:
Basic fibroblast growth factor (bFGF or Heparin-binding growth factor 2) is a single-chain polypeptide growth factor involved in wound healing, angiogenesis and embryonic development. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. bFGF binds to heparin and signals through four receptor tyrosine kinases, FGFR1, FGFR2, FGFR3 and FGFR4. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor. Recombinant Human FGF-b (FGF-2) produced in E. coli is a single non-glycosylated polypeptide chain containing 154 amino acids with a MW of 17.2 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.01 ng/ug or <0.1 EU/ug.
Applications: E, WB
Application Notes:
rH-FGF-b is fully biologically active when compared to standards. The ED50 measured in a cell proliferation assay using NIH-3T3 fibroblasts is 0.1-0.14 ng/ml.
Accession No.: P09038
MW: 17200 Da
Sequence:
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
Purity:
Purity > 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 50 ug protein lyophilized in 10 mM sodium phosphate pH8.0. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Signal Transduction, Cancers, Cell Motility, Cell Growth and Death
Alternate Names: Heparin-binding growth factor 2, HBGF-2, Basic fibroblast growth factor, BFGF, Prostatropin, FGF2, FGFB, FGF-2, FGF-b 154Description:
Basic fibroblast growth factor (bFGF or Heparin-binding growth factor 2) is a single-chain polypeptide growth factor involved in wound healing, angiogenesis and embryonic development. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. bFGF binds to heparin and signals through four receptor tyrosine kinases, FGFR1, FGFR2, FGFR3 and FGFR4. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor. Recombinant Human FGF-b (FGF-2) produced in E. coli is a single non-glycosylated polypeptide chain containing 154 amino acids with a MW of 17.2 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.01 ng/ug or <0.1 EU/ug.
Applications: E, WB
Application Notes:
rH-FGF-b is fully biologically active when compared to standards. The ED50 measured in a cell proliferation assay using NIH-3T3 fibroblasts is 0.1-0.14 ng/ml.
Accession No.: P09038
MW: 17200 Da
Sequence:
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
Purity:
Purity > 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 50 ug protein lyophilized in 10 mM sodium phosphate pH8.0. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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