bFGF mouse active recombinant protein
€295.00
In stock
SKU
600182
Protein Family: Cytokines, CAM ligands
Pathway and Disease: Signal Transduction, Cancers, Cell Motility, Cell Growth and Death
Alternate Names: Heparin-binding growth factor 2, HBGF-2, Basic fibroblast growth factor, BFGF, FGF2, FGF-2Description:
Basic fibroblast growth factor (bFGF or Heparin-binding growth factor 2) is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. Several different forms of the protein exist, ranging from 18-24 kDa in size due to the use of alternative start sites within the FGF-2 gene. It has a 55 percent amino acid residue identity to FGF-1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor. Recombinant Mouse basic FGF (FGF-2) produced in E. coli is a non-glycosylated single polypeptide chain containing 145 amino acids with a MW of 16,320 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
The ED50, calculated by the dose-dependent proliferation of BALB/3T3 cells is 0.1 ng/ml.
Accession No.: P15655
MW: 16320 Da
Sequence:
MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 50 ug lyophilized protein. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Signal Transduction, Cancers, Cell Motility, Cell Growth and Death
Alternate Names: Heparin-binding growth factor 2, HBGF-2, Basic fibroblast growth factor, BFGF, FGF2, FGF-2Description:
Basic fibroblast growth factor (bFGF or Heparin-binding growth factor 2) is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. Several different forms of the protein exist, ranging from 18-24 kDa in size due to the use of alternative start sites within the FGF-2 gene. It has a 55 percent amino acid residue identity to FGF-1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor. Recombinant Mouse basic FGF (FGF-2) produced in E. coli is a non-glycosylated single polypeptide chain containing 145 amino acids with a MW of 16,320 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
The ED50, calculated by the dose-dependent proliferation of BALB/3T3 cells is 0.1 ng/ml.
Accession No.: P15655
MW: 16320 Da
Sequence:
MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 50 ug lyophilized protein. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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