biotin (Vitamin B7 or Vitamin H) (clone SPM375), anti-all species
€0.00
In stock
SKU
ARP-24-MSM1X-400
Catalog Number: 24-MSM1X-400
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1
Decription:
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Synonyms: Vitamin B7 or Vitamin H
Clone: SPM375
Isotype: Mouse IgG1
Immunogen: Biotinylated sheep immunoglobulin
Reactivity: all species
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Confocal Microscopy
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
In situ Nucleic Acid Hybridization
Western Blotting
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues) Optimal dilution for a specific application should be determined.
Reactivity: Biotinylated proteins in solution or on tissues
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Synonyms: Vitamin B7 or Vitamin H
Clone: SPM375
Isotype: Mouse IgG1
Immunogen: Biotinylated sheep immunoglobulin
Reactivity: all species
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Confocal Microscopy
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
In situ Nucleic Acid Hybridization
Western Blotting
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues) Optimal dilution for a specific application should be determined.
Reactivity: Biotinylated proteins in solution or on tissues
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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