Blasticidin S Deaminase Blocking Peptide
€155.00
In stock
SKU
ECM-BX1235
Background:
Retroviral vectors are commonly used for gene expression in vitro and in vivo. The structure of these vectors usually consists of one gene of interest and a selectable marker gene. Fast selection without damaging cells is a critical step for significant gene expression. Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin. A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin. This selectable marker allows selection of stable mammalian cell lines in as little as one week. Catalog# BP1231 is a Bsd antibody that can detect Bsd expression in cells transfected with blasticidin-selectable constructs. This antibody is useful for detection of successful gene transfer in constructs that utilize blasticidin selection.
Sequence: A synthetic peptide corresponding to amino acid residues in the N-terminal region of Blasticidin S Deaminase from Aspergillus Terrus.
Specificity: The peptide is specifically recognized by anti-Blasticidin S Deaminase antibody (BP1231) in ELISA, and is recommended for use in blocking BP1231 reactivity in Western blot and immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Retroviral vectors are commonly used for gene expression in vitro and in vivo. The structure of these vectors usually consists of one gene of interest and a selectable marker gene. Fast selection without damaging cells is a critical step for significant gene expression. Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin. A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin. This selectable marker allows selection of stable mammalian cell lines in as little as one week. Catalog# BP1231 is a Bsd antibody that can detect Bsd expression in cells transfected with blasticidin-selectable constructs. This antibody is useful for detection of successful gene transfer in constructs that utilize blasticidin selection.
Sequence: A synthetic peptide corresponding to amino acid residues in the N-terminal region of Blasticidin S Deaminase from Aspergillus Terrus.
Specificity: The peptide is specifically recognized by anti-Blasticidin S Deaminase antibody (BP1231) in ELISA, and is recommended for use in blocking BP1231 reactivity in Western blot and immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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