BMP-4 monoclonal, anti-human

Source:
Produced in mice using highly pure (>98%) recombinant human BMP-4 as the immunizing antigen. This IgG2b kappa antibody was purified from cell culture by Protein G affinity chromatography.

Western Blot:
To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 2.0-4.0 ng/lane, under non-reducing conditions.

Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 2000 pg/ml of recombinant human BMP-4 when used in conjunction with compatible secondary reagents.

Immunohistochemistry:
This antibody stained HepG2 cells. The primary antibody was incubated at 3.0 μg/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human BMP-4 (5.0 ng/ml), a concentration of 0.4-0.6 μg/ml of this antibody is required.

Formulation: A sterile filtered antibody solution was lyophilized from PBS.

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.