BOB1 polyclonal, anti-mouse, rat

Quantity: 100 µg

Background:
POU2AF1(POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al.(2002) reported that OCAB is essential for V(D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by directly enhancing Ig-kappa gene transcription in vivo. Siegel et al.(2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.

Description:
Rabbit IgG polyclonal antibody for POU domain class 2-associating factor 1 (POU2AF1) detection. Tested with WB, IHC-P in Mouse, Rat.

Synonyms:
B cell Oct binding protein 1 antibody, B cell specific coactivator OBF 1 antibody, B cell specific coactivator OBF1 antibody, B-cell-specific coactivator OBF-1 antibody, BOB 1 antibody, BOB-1 antibody, OBF 1 antibody, OBF1 antibody, OBF1_HUMAN antibody, OCA B antibody, OCA-B antibody, OCAB antibody, OCT binding factor 1 antibody, OCT-binding factor 1 antibody, POU domain class 2 associating factor 1 antibody, POU domain class 2-associating factor 1 antibody, POU2AF1 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of mouse BOB1(238-256aa EEEESNTYELNHTLSVEGF), different from the related rat sequence by one amino acid, and from human sequence by four amino acids.A synthetic peptide corresponding to a sequence at the C-terminus of mouse BOB1(238-256aa EEEESNTYELNHTLSVEGF), different from the related rat sequence by one amino acid, and from human sequence by four amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: mouse, rat

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.