Bovine 70kDa Heat Shock Protein 1A (HSPA1A) Detection Kit

Use: Used to detect the concentration of bovine 70kDa heat shock protein 1A (HSPA1A) in samples such as serum, plasma, and cell culture supernatant.Specificity: This kit uses double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In the microwell ELISA plate pre-coated with anti-bovine 70kDa heat shock protein 1A (HSPA1A) antibody (solid phase antibody), add bovine 70kDa heat shock protein 1A (HSPA1A) calibrator and the sample to be tested, then add biotin-labeled antibody, incubate and fully wash, then add HRP-coupled avidin, incubate and fully wash to remove unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid phase surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which eventually turns into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on the ELISA instrument. The absorbance (OD value) is positively correlated with the concentration of bovine 70kDa heat shock protein 1A (HSPA1A) in the sample to be tested. By fitting the calibrator curve, the concentration of bovine 70kDa heat shock protein 1A (HSPA1A) in the sample can be calculated.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.