Bovine Albumin polyclonal HRP, anti-bovine

Quantity: 200 µl

Background:
Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal bovine serum and purified bovine albumin. One characteristic precipitin line is obtained against pooled normal bovine serum using different antigen/antibody concentration ratio's. Precipitin lines against normal bovine serum and purified bovine albumin give a reaction of full identity. In enzyme-immunocytochemical and immunohistochemical staining for the detection of albumin, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure albumin in bovine serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:10,000.

Isotype: Rabbit Polyclonal

Source: Purified albumin isolated from pooled bovine serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Peroxidase-coupled purified hyperimmune rabbit IgG, in phosphate buffered saline (PBS, pH 7.2).

Reactivity: bovine

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in double radial immunodiffusion with the following results: Cat + Chicken - Dog + Duck - Goat ++ Guinea Pig � Hamster + Horse + Human + Monkey + Mouse � pigeon - Rabbit - Rat � Sheep ++ Swine + A negative result in this test does not exclude some cross-reaction in more sensitive techniques.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.