Bovine/Horse/Sheep E2 (Estradiol) ELISA Kit
Catalog Number: ACE-E0116
Size: 96 tests/kit
Detection Range: 9.37—600 pg/mL
Sensitivity: 5.63 pg/mL
Datasheet
Request Information
Price on request
Size: 96 tests/kit
Detection Range: 9.37—600 pg/mL
Sensitivity: 5.63 pg/mL
Datasheet
Request Information
Price on request
X
You are enquiring about Bovine/Horse/Sheep E2 (Estradiol) ELISA Kit
Description:
This ELISA kit can be applied to the in vitro quantitative determination of E2 concentrations in serum， plasma.
Assay Principles: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. During the reaction, E2 in the sample or standard competes with a fixed amount of E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to E2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Species reactivity:
Bovine/Horse/Sheep
Sample Type: serum, plasma
Storage instruction: -20˚C and 4˚C for 6 months
This ELISA kit can be applied to the in vitro quantitative determination of E2 concentrations in serum， plasma.
Assay Principles: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. During the reaction, E2 in the sample or standard competes with a fixed amount of E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to E2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Species reactivity:
Bovine/Horse/Sheep
Sample Type: serum, plasma
Storage instruction: -20˚C and 4˚C for 6 months
| Is Featured? | No |
|---|
Write Your Own Review