Bovine NAGase (N-Acetyl Beta-D-Glucosaminidase) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acetyl Beta-D-Glucosaminidase(NAGase). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Acetyl Beta-D-Glucosaminidase(NAGase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Acetyl Beta-D-Glucosaminidase(NAGase), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Acetyl Beta-D-Glucosaminidase(NAGase) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase, Kidney biomarker

Alternative Names:
MGEA5, MEA5, NCOAT, Hexosaminidase C, N-Acetylhexosaminidase, Hyaluronidase, Meningioma-Expressed Antigen 5, Nuclear Cytoplasmic O-GlcNAcase And Acetyltransferase

Sample Type:
Urine

Assay Type: Sandwich

Standard: 100 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only