Bovine serum proteins polyclonal, anti-bovine

Quantity: 200 µl

Background:
In immunoelectrophoresis against pooled serum and enriched serum proteins fractions precipitation can be observed of not less than 11 individual proteins components. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony) to identify the serum protein pattern, or the presence or absence of an individual component. To evaluate the purity of an isolated serum protein including immunoglobulins. Since immunoprecipitation depends on a correct antigen/antibody concentration ratio (zone of equivalence) in the gel medium, the protein analysis by immunoelectrophoresis of serum or any other biological fluid or protein fraction should include different proportions of the reactants. It is not possible to obtain an optimal protein pattern in a single analysis. The electroendosmosis effect of different types of agar on proteins with a different net charge can be used to optimize the resolution power of the test system. Agar Nordic Nr. 2 contains sufficient positively charged ions to optimize the resolution of the proteins in the beta-gamma regions, while the alpha regions will become more dense. Highly purified agar (Agar Nordic nr. 1) with low electroendosmosis favours the resolution of the proteins in the alpha regions, while the major components in the betagamma region can still be identified.

Isotype: Sheep Polyclonal

Source: Pooled whole bovine serum and partly purified serum fractions. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: bovine

Cross Reactivity:
Inter-species cross reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiserum has not been adsorbed for such cross reactivity. Consequently it is not species-specific.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.