Bovine Ubiquitin (Ub) Detection Kit

Use: Used to detect the concentration of bovine ubiquitin (Ub) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled bovine ubiquitin (Ub) and purified anti-bovine ubiquitin (Ub) antibodies are coated on microplates. In the competitive inhibition reaction, a certain amount of solid-phase antibodies inhibit the competitive reaction with biotin-labeled bovine ubiquitin (Ub) and non-labeled antigens (calibrators or specimens). The binding amount of the antibody and biotin-labeled bovine ubiquitin (Ub) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of the antibody and biotin-labeled bovine ubiquitin (Ub), and vice versa. After the reaction is balanced, a solid-phase antibody-biotinylated bovine ubiquitin (Ub) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated bovine ubiquitin (Ub)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of bovine ubiquitin (Ub) increases, the OD value gradually decreases, showing a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of bovine ubiquitin (Ub) in samples.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.