BRCA2 Polyclonal Antibody

Background:
Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
BRCA 2, BRCC 2, Breast and ovarian cancer susceptibility gene early onset antibody, BROVCA2, FACD, FAD 1, FANCB, GLM3

Immunogen: Recombinant Human Breast cancer type 2 susceptibility protein

Swissprot: P51587

Calculated MW: 384 kDa
Observed MW: 384 kDa

Purification Method: Antigen Affinity Purification

Buffer: PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.

Concentration: 1 mg/mL
Dilution: WB 1:1000-1:3000 IHC 1:100-1:200 IF 1:100-1:400

Storage:
Store at -20°C. Avoid freeze / thaw cycles.