BrdU (clone MoBU-1), anti-all species

Source:
A BALB/c mouse was immunized with a conjugate of bromodeoxyrudine and Helix pomatia hemocyanin (HPH).The conjugate was prepared using periodate oxidation.
Fusion partner: P3-X63-Ag8.653.

Specifications:
MoBU-1 is reactive with cells containing incorporated 5-bromodeoxyuridine (BrdU) showing a clear, nucleus confined, speckled pattern. BrdU is an analogue of thymidine and can be introduced to proliferating cells in vitro or in vivo, which in turn incorporate BrdU into the DNA during S phase, prior to cell division. Immunocytochemical staining with MoBU-1 will show the degree of proliferation, detecting nucleated cells which have incorporated BrdU in place of thymidine into their DNA.

Species reactivity:
Positive: all species.

Applications:
Immunocytochemistry on frozen and paraffin sections. Immunofluorescence tests.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: HCl/protease

Positive control: Cultured cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

References:
- Harms et al. Acta Histochemica, Suppl. Band 36, 353-359 (1988).
- Arras et al. J Clin Invest.101(1), 40-50 (1998)