BTK polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
BTK, also known as Bruton's tyrosine kinase, is an enzyme that in humans is encoded by the BTK gene. It is mapped to Xq22.1. BTK plays a crucial role in B cell maturation as well as mast cell activation through the high-affinity IgE receptor. BTK contains a PH domain that binds phosphatidylinositol (3,4,5)-trisphosphate (PIP3). PIP3 binding induces BTK to phosphorylate phospholipase C, which in turn hydrolyzes PIP2, a phosphatidylinositol, into two second messengers, inositol triphosphate (IP3) and diacylglycerol (DAG), which then go on to modulate the activity of downstream proteins during B-cell signalling. This gene also regulates both TLR9 activation and expression in B lymphocytes and is necessary for inhibitory cytokine expression.

Description:
Rabbit IgG polyclonal antibody for Tyrosine-protein kinase BTK (BTK) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
Agammaglobulinaemia tyrosine kinase antibody, AGMX 1 antibody, AGMX1 antibody, AT antibody, ATK antibody, B cell progenitor kinase antibody, B-cell progenitor kinase antibody, BPK antibody, Bruton agammaglobulinemia tyrosine kinase antibody, Bruton tyrosine kinase antibody, Bruton’s Tyrosine Kinase antibody, Btk antibody, BTK_HUMAN antibody, IMD 1 antibody, IMD1 antibody, MGC126261 antibody, MGC126262 antibody, OTTHUMP00000063593 antibody, PSCTK 1 antibody, PSCTK1 antibody, Tyrosine protein kinase BTK antibody, Tyrosine-protein kinase BTK antibody, XLA antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
E.coli-derived human BTK recombinant protein (Position: A2-P340). Human BTK shares 98% amino acid (aa) sequence identity with mouse BTK.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.