Bub1 polyclonal, anti-human, rat

Quantity: 100 µg

Background:
BUB1, also known as mitotic checkpoint serine/threonine kinase, is an enzyme that in humans is encoded by the BUB1 gene. It is mapped to 2q13. BUB1 is first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of BUB1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence. The protein kinase BUB1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase.

Description:
Rabbit IgG polyclonal antibody for Mitotic checkpoint serine/threonine-protein kinase BUB1 (BUB1) detection. Tested with WB in Human, Rat.

Synonyms:
Bub1 antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody, BUB1 mitotic checkpoint serine/threonine kinase antibody, BUB1, S. cerevisiae, homolog of antibody, BUB1_HUMAN antibody, BUB1A antibody, BUB1L antibody, Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody, Budding uninhibited by benzimidazoles 1 homolog antibody, Budding uninhibited by benzimidazoles 1, S. cerevisiae, homolog of antibody, hBUB1 antibody, Homolog of mitotic checkpoint gene BUB1 antibody, Mitotic checkpoint gene BUB1 antibody, Mitotic checkpoint serine/threonine protein kinase BUB1 antibody, Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody, Mitotic spindle checkpoint kinase antibody, Putative serine/threonine protein kinase antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
E.coli-derived human Bub1 recombinant protein (Position: V731-K1085). Human Bub1 shares 81% amino acid (aa) sequence identity with mouse Bub1.E.coli-derived human Bub1 recombinant protein (Position: V731-K1085). Human Bub1 shares 81% amino acid (aa) sequence identity with mouse Bub1.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.