Butyrophilin polyclonal, anti-human, bovine, rat, mouse

Quantity: 25 µl

Background:
Butyrophilin (Btn) genes constitute a subgroup of at least 10 genes in the Ig superfamily identified in human, mouse, cow, goat and other species. The eponymous Btn gene (BTN1A1 in humans; Btn1a1 in mouse) is highly expressed in the secretory epithelium of the mammary gland during lactation. Other homologues are predominantly expressed in skeletal muscle and the intestine and erythroid cells. In contrast, BTN2A1 and 2 and BTN3A1, 2, and 3 are widely expressed in many tissues, suggesting that the structural domains of Btn proteins may have both universal and tissue-specific functions.

Isotype: Guinea pig Polyclonal

Immunogen:
Butyrophilin purified from bovine milk fat globule membrane

Form: Stabilized Serum

Specificity:
The antiserum reacts specifically with butyrophilin, a unique 67kDa polypeptide present only in lactating mammary gland epithelium and the milk fat globule membrane (MFGM). No cross-reaction with other MFGM proteins.

Reactivity: human, bovine, rat, mouse

Applications: Immunohistochemistry, suitable for frozen & paraffin embedded tissue after microwave treatment, Immunoblotting (Western, ECL)

Working DIlution: Immunohistochemistry: 1:100 to 1:200; Western Blotting: 1:2,000 (ECL)

Storage: 2-8C

References:
- Franke, WW. et al.(1981) J Cell Biol 89:485-494
- Ogg, SL. et al. (2004) Proc Natl Acad Sci USA 101: 10084-10089