c-Myb (Phospho-S11) polyclonal, anti-human

Background:
The Myb family of transcription factors regulates proliferation, differentiation and apoptosis of hematopoletic cells. The avian myeloblastosis viral (v-Myb) protein is nuclear and binds to specific DNA sequences. The gene encoding for v-Myb is oncogenic, causing monoblastic leukemia and transforming myelomonocytic cells by deregulating the expression of specific target genes. v-Myb functions as a transcriptional activator, and it can repress biologically relevant genes such as Ets-2, which promotes macrophage differentiation. The proto-oncogene c-Myb encodes for a nuclear protein that plays a role in transcriptional regulation and may be essential for hematopoietic cell proliferation. A-Myb is also member of the Myb family that is expressed in proliferating B cell centroblasts. Transgenic mice overexpressing A-Myb posses enhanced hyperplasia of the lymph nodes.

Alternative Name:
MYB, C myb, C myb protein, Cmyb, MYB, Myb proto oncogene, V myb myeloblastosis viral oncogene homolog,

Application Dilution:
WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: c-Myb (Phospho-S11) polyclonal antibody detects endogenous levels of c-Myb protein only when phosphorylated at S11.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Ser11 of human V-Myb+C-Myb.

MW: 72/75 kDa

Swiss-Prot: P10242(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.