C-RAF (Phospho-Ser642) polyclonal, anti-human, mouse, rat

Specificity:
C-RAF (Phospho-Ser642) Antibody detects endogenous levels of RAF1 only when phosphorylated at Ser642.

Synonyms: C-RAF, C-Raf, CRAF, kinase Raf1, Protein kinase raf 1, RAF proto-oncogene serine/threonine-protein kinase, Raf proto-oncogene serine/threonine-protein kinase, RAF-1, Raf-1, RAF1, Similar to murine leukemia viral (Phospho-V-raf-1) oncogene homolog 1

Immunogen:
The antiserum was produced against synthesized phospho-peptide derived from human C-RAF around the phosphorylation site of Ser642.

Immunogen Sequence:
Phospho

Modification Sites: H:S642, M:S642, R:S642

MW (kDa): 73 kDa

NCBI Gene Symbol: RAF1

Database Links:
Gene ID: 5894
Swiss Prot No.: P04049
OMIM No.: 164760/611553/611554
Unigene No.: Hs.159130

Application Dilutions: WB: 1:500~1:1000, ELISA: 1:10000

Purification:
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Physical Format:
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/ml

Storage: Stable at -20°C for at least 1 year.