C14orf169 Polyclonal Antibody

Background:
Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.

Research Area: Epigenetics and Nuclear Signaling

Synonyms:
60S ribosomal protein L8 histidine hydroxylase, C14orf169, Chromosome 14 open reading frame 169 , Histone lysine demethylase NO66, hsNO66, Hypothetical protein FLJ21802, Lysine-specific demethylase NO66, MAPJD, MYC associated protein with JmjC domain, NO66, NO66, Nucleolar protein 66, Ribosomal oxygenase NO66, ROX, Up regulated in lung cancer 2, URLC2

Immunogen: Synthetic peptide of human C14orf169

Swissprot: Q9H6W3
Gene Accession: NP078920

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 0.8 mg/mL
Dilution: IHC 1:30-1:150, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.