C1q polyclonal FITC, anti-human

Quantity: 200 µl

Background:
Tested in immunoelectrophoresis and double radial immunodiffusion against normal human serum, a single precipitin line is obtained which gives a reaction of full identity with the isolated immunogen. The product does not react with any other proteins component of human serum or plasma. To identify C1q and C1q containing complexes at the cellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. Determinations of individual C components can be very useful in defining the exact location of a defect.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.Working dilutions are usually between 1:20 and 1:80.

Isotype: Goat Polyclonal

Source: C1 component of human complement is a complex of three glycoproteins: C1q, C1r and C1s. C1q has a molecular weight of 492.000 and is present in plasma in an average concentration of 80 ?g/ml. It consists of a complex of 18 polypeptide chains of three different types, A, B and C; 6 of each type. The subcomponent C1q is responsible for the binding of C1q to immuno-complexes an membranes via the Fc portion of the immunoglobulin. The subunit C1q is isolated as a homogenous protein for use in antiserum production. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Fluorochrome-coupled purified hyperimmune IgG, in phosphate buffered saline (PBS, pH 7.2)

Specificity:
Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat anti-Serum to C1q fragment of Human complement

Reactivity: human

Cross Reactivity:
The antiserum does not cross-react with any other component of Human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence

Storage: Store at 2-8C, or in small aliquots at -20C.