C3c polyclonal, anti-monkey

Quantity: 200 µl

Background:
In immunoelectrophoresis against fresh monkey serum, a single precipitin line is obtained in the beta- 1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of monkey serum or plasma. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of complement C3c or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.

Isotype: Goat Polyclonal

Source: C3c is the major fragment resulting from the C3 cleavage by C3 convertase and factor i. It is composed of an intact beta chain bound to two fragments of the alpha chain. The protein is isolated and purified from pooled normal monkey serum by precipitation techniques, followed by chromatographical methods.Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Specificity:
Precipitating polyclonal Goat anti-Serum to C3c subunit of Monkey complement C3

Reactivity: Monkey

Cross Reactivity:
The antiserum does not cross-react with any other component of Monkey plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. In addition to a clear reactivity with C3c of other old-world Monkeys (Cercopithecus, Cynomolgus and Baboon), this antiserum shows also a fair amount of cross-reactivity to C3C of other species, including Chimpanzee and man.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.