C3c polyclonal biotin, anti-rat

Quantity: 200 µl

Background:
In immunoelectrophoresis against fresh rat serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of rat serum or plasma. The biotin immunoconjugate to rat C3c is used to determine the presence and pattern of C3 in tissue lesions using immunohistochemical staining techniques. Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. In ELISA and Western blotting to identify rat C3c in serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; In ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:5,000.

Isotype: Goat Polyclonal

Source: C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. C3c is isolated and purified from pooled normal rat serum.Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Biotin-coupled purified hyperimmune IgG fraction, in phosphate buffered saline (pH7.2).

Specificity:
Biotin-conjugated IgG fraction of polyclonal Goat anti-Serum to C3c fragment of Rat complement factor C3.

Reactivity: rat

Cross Reactivity:
The antiserum does not cross-react with any other component of Rat plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.