C3d polyclonal, anti-human

Quantity: 200 µl

Background:
Tested in immunoelectrophoresis and double radial immunodiffusion against normal human serum, a single precipitin line is obtained and giving a reaction of full identity with the purified immunogen. The antiserum is also reacting with fragments bearing common determinants: C3, C3b, C3bi. The product does not react with any other proteins component of human serum or plasma. In supernatants of sera containing 10% polyethyleneglycol only a reaction with C3d has been obtained. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of complement C3d or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.

Isotype: Goat Polyclonal

Source: C3d is a component of complement. Cell-bound C3b may undergo limited proteolysis to release the fragment C3c and retain C3d on the membrane. The protein is isolated and purified from pooled normal human serum by precipitation techniques, followed by chromatographical methods.Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, stable whole serum, no preservative.

Specificity:
Precipitating polyclonal Goat anti-Serum to C3d subunit of Human complement C3

Reactivity: human

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.

Applications: Immunoprecipitation

Storage: Store at 2-8C, or at -20C (aliquots).