C4d polyclonal, anti-human

Quantity: 250 µl

Description:
This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluste exist, such that individuals may have 1, 2, or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene.

Isotype: Rabbit polyclonal

Immunogen:
Synthetic peptide specific for human C4d conjugated to KLH (keyhole limpet hemocyanin)

Form: Affinity Purified IgG, in PBS, containing Stabilizer and 0.02% sodium azide

Reactivity: human, tested so far

Applications: Immunohistochemistry, suitable for frozen section and paraffin embedded tissue. Pretreatment of sample is highly recommended for antigen retrieval.

Working DIlution: min.1: 50 (see protocol)

Storage: 2-8C for immediate use, or at -20C (aliquot). AVOID repeated freezing and thawing