C5 polyclonal FITC, anti-human

Quantity: 200 µl

Background:
Tested in immunoelectrophoresis and double radial immunodiffusion against normal human serum, a single precipitin line is obtained which gives a reaction of full identity with the isolated immunogen. The product does not react with any other proteins component of human serum or plasma. The fluorescent immunoconjugate to human C5 is used to determine the presence and pattern using immunohistochemical staining techniques. Determinations of individual C components can be very useful in defining the exact location of a defect.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.Working dilutions are usually between 1:10 and 1:40.

Isotype: Goat Polyclonal

Source: C5 is a component of the complement system. It has a molecular weight of 190,000. The activated protein forms with the complement factors C6-C9 the membrane attack-complex. C5 is isolated and purified from pooled normal human serum.Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Fluorochrome-coupled purified hyperimmune IgG, in phosphate buffered saline (PBS, pH 7.2)

Specificity:
Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat anti-Serum to C5 fragment of Human complement

Reactivity: human

Cross Reactivity:
The antiserum does not cross-react with any other component of Human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence

Storage: Store at 4C, or in small aliquots at -20C.