CAD Polyclonal Antibody

Background:
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.

Research Area: Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Metabolism

Synonyms:
Aspartate carbamoyltransferase, Aspartate transcarbamylase, CAD protein, CAD trifunctional protein, Carbamoyl phosphate synthetase 2, Carbamoyl-phosphate synthetase 2 aspartate transcarbamylase and dihydroorotase, CPSase ATCase DHOase, Dihydroorotase, Glutamine dependent carbamoyl phosphate synthase, Multifunctional protein CAD

Immunogen: Synthetic peptide of human CAD

Swissprot: P27708
Gene Accession: NP004332

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 0.7 mg/mL
Dilution: IHC 1:40-1:200, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.