CAD Polyclonal Antibody

Background:
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.

Research Area: Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Metabolism

Synonyms:
CAD, CDG1Z, EIEE50, GATD4

Immunogen: Recombinant fusion protein of human CAD (NP_004332.2).

Swissprot: P27708
Gene ID: 790

Calculated MW: 242 kDa
Observed MW: 243 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000 IHC 1:50-1:100 IF 1:50-1:200"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.