Caldesmon monoclonal, anti-human, mouse, rat

Background:
Caldesmon is a protein that in humans is encoded by the CALD1 gene.Caldesmon (CDM) is a potential actomyosin regulatory protein found in smooth muscle and nonmuscle cells. Domain mapping and physical studies suggest that CDM is an elongated molecule with an N-terminal myosin/calmodulin-binding domain and a C-terminal tropomyosin/actin/calmodulin-binding domain separated by a 40-nm-long central helix.1The high molecular weight caldesmon (h-CaD) is predominantly expressed in smooth muscles, whereas the low molecular weight caldesmon (l-CaD) is widely distributed in nonmuscle tissues and cells.2The conserved domain of this protein possesses the binding activities to Ca++-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca++-dependent inhibition of smooth muscle contraction.

Immunogen:
Asynthetic peptide within C-terminal human Caldesmon.

Recommended Application:
Western Blot, WB(1:500-1:1, 000); Immunohistochemistry, IHC-P (1:20-1:100); Immunocytochemistry, ICC/IF (1:20-1:100); Flow Cytometry, Flow-Cyt (1:20-1:50)

Product Form: Liquid

Purification: Protein A affinity purified

Storage Buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction:
Store at 4℃ after thawing (1 week). Aliquot and store at -20℃ for long term (at least one year). Avoid repeated freeze and thaw cycles.